For further confirmation, we also examined expression changes for Jagged1 and Notch1 and the panel of Notch target genes, Hes1, Hey1, Hey2, and Hrt2, by RT-qPCR following PMZ and S3I-201 treatment of LN18 cells (Figure 2(B, D), respectively) and also for other glioblastoma cells (Figure S1). Here, HES1 is linked to glioblastoma.