We used mouse AK organoids established from colonic tumors developed in mice carrying Villin-CreERT2/+, lsl-KrasG12D/+, and ApcΔ716/+ alleles (Sakai et al., 2017), since APC and KRAS mutations were observed in >80% and >40% of CRC, respectively. The gene discussed is APC; the disease is colonic neoplasm.