Adoptive transfer of wild type CD25highCD127low/−Foxp3+CD4+Treg cells efficiently ameliorated the exacerbated inflammatory response of colitic Lgals1−/− mice whereas galectin-1 null Treg cells were unable to ameliorate the exacerbated inflammatory intestinal inflammation in colitic Lgals1−/− mice further supporting the pivotal role played by galectin-1 of CD25highCD127low/−Foxp3+CD4+ Treg cells in modulating the severity of DSS-induced colitis. This evidence concerns the gene CD4 and colitis.