We also detected the IFN-γ, TNF-α and IL-6 in serum in the above GVHD models and found that the concentrations of IFN-γ, TNF-α and IL-6 reduced in recipients received Lyg1-/- donor T cells compared with recipients received Lyg1-/- donor T cells, especially for IFN-γ (Figure S5). Here, IL6 is linked to graft versus host disease.