Patel et al. (2015) examined the impact of the loss of Tsc1 on the function on surrounding normal cells using the in vivo mouse embryo-neural tube model. Based on their cell phenotype results, they posited that cells without Tsc1 might secrete EVs that alter surrounding cells with a preserved Tsc1 locus such that mutant cells disseminate the TSC disease phenotype. However, they did not isolate EVs (Patel et al., 2015). This evidence concerns the gene TSC1 and tuberous sclerosis.