PRF1 and stroke disorder: As documented here using Prf1-EGFP transgenic mice, the kinetics of perforin mainly induced direct cytotoxic effects of brain-resident microglia and invading immune cells, and perforin did not have an effect on stroke outcomes in Prf1–/– mice subjected to dMCAO; however, the neurological deficits and histological outcomes paralleled the change in neurogenesis.