In the third stage, we applied the best protocol to sequence six single cells each of eight different cell lines (fibroblasts, T-cells, monocytes, macrophages, lymphoblasts, colorectal adenocarcinoma, lung cancer, and hepatocellular carcinoma) and 67 single EpCAM positive CTCs from the blood of seven SCLC patients. This evidence concerns the gene EPCAM and hepatocellular carcinoma.