We analyzed a total of 421,059 cells from 12 immune cell types (Supplementary Table 3, Supplementary Fig. 1) and found significant increases in the proportion of CD4 T Cells, CD8 T Cells, cDCs and NK cells in the disease groups, most notably in COPD samples (Fig. 4a). This evidence concerns the gene CD8A and chronic obstructive pulmonary disease.