The population of CD80+CD86+ cells in DiD positive macrophages of Oxa(IV)@Lip@M were counted to be ~70%, which was similar to that of LPS treated M1 cells, while blank BMMs only counted to be ~37% (Fig. 4m, n), indicating that the drug loaded macrophages could retain their M1 phenotype in the tumor tissue. Here, CD80 is linked to neoplasm.