IHC using anti-CD3, anti-CD8, and anti-CD20 antibodies on 3 FFPE tumor samples per patient, biopsied at distant anatomic sites, showed heterogeneous infiltration of T and B cells in T199LE patient and to a lesser extent in T225LE and T227LE patients (Additional file 2: Figure S14). This evidence concerns the gene CD8A and neoplasm.