TP53 and liver cancer: As nonpathogenic adeno-associated virus infects both dividing and nondividing cells with mild immunogenicity [48, 49], Wang G et al. successfully induced liver cancer by mutating the liver of Cre-inducible Cas9 mice with an adeno-associated virus as a carrier to deliver the sgRNA library aimed at possible tumor suppressor genes, such as Trp53, Setd2, Cic, and Pik3r1 [50].