As shown in Table 1, in both T cells and NK cells, cells with GPC3-O4-CAR structure secreted more IFN-γ than those with GPC3-CD8-CAR and GPC3-ori-CAR, indicating that immune cells expressing GPC3-O4-CAR could be better activated by GPC3 on the surface of cancer cells. This evidence concerns the gene CD8A and cancer.