ANXA5 and cardiovascular disorder: Additionally, we used a ubiquitously expressed secreted Annexin-V line, which binds with high affinity to the phosphatidylserine expressed on the outer surface of apoptotic cells (Tg(tbp:GAL4);(UAS:secA5-YFP))30; Figure IIA and IIB in the Data Supplement), but also on populations of EVs, including those relevant in cardiovascular disease.45,46 As with the actb2 line, we observed Annexin-V labeled particles in both the peripheral circulation and pericardial space of larval zebrafish (Figure IIC and IID and Movie III in the Data Supplement).