LGALS1 and gastric cancer: To further clarify the origin of GAL-1/LGALS1, we cultured CAFs from human GC tissue, and the GC cell lines (SGC-7901, AGS, BGC-823, and MGC-803) in vitro and investigated the GAL-1 levels in all cells using WB, which confirmed strong GAL-1 expression in CAFs, but lower levels in GC cells (Figure 2A).