To analyze whether YKL-40 affects cell invasion and metastasis through EMT in bladder cancer, we detected the EMT related genes and transcription factors in SVHUC1 overexpressing YKL-40 and T24 cells down regulating YKL-40 expression, such as adhesion markers E-cadherin, Twist, Snail, Slug and N-cadherin, Vimentin. Here, TWIST1 is linked to urinary bladder cancer.