Finally, after removing the genes whose knockout was toxic in major human normal tissue cells, we filtered out four genes, SOAT1, CRLS1, and ACACB, which are essential for all three subtypes of ccRCC, showing the potential to be used as drug targets for the majority of patients with ccRCC regardless of specific molecular subtype, as well as GPD2 which is essential for subtype 1 specifically. This evidence concerns the gene GPD2 and nonpapillary renal cell carcinoma.