We found that inhibition of the electron transport chain with a low dose of rotenone (50 nM) increased ROS production (Figure 5(g)) as well as downregulated expression of the epithelial protein marker E-cadherin and upregulated expression of the mesenchymal protein markers Vimentin and N-cadherin (Figure 5(h), Fig. S5(b)) which also confirmed that mitochondrial dysfunction and ROS production were necessary for EMT process in glioblastoma cells. The gene discussed is VIM; the disease is glioblastoma.