Immunofluorescence staining confirmed the induction of cell neurite outgrowth in the presence of GFAP astrocyte marker and differentiation indicator S100β, both in LGG2 control and h-TERT-LGG2 cells under NGF treatment (Figure 5B), demonstrating that hTERT-transduced glioma cells retained the susceptibility to NGF-mediated differentiation shown by the parental counterpart. This evidence concerns the gene S100B and central nervous system cancer.