This study is the first to show how NSCLC PD-L1 scoring using free open source software compares with the range of manual scores generated by a group of pathologists: QuPath automated PD-L1 scores showed agreement with average manual scores equivalent to that of individual pathologists, such that the overall accuracy of automated scoring could be considered comparable to that of individual pathologists. The gene discussed is CD274; the disease is non-small cell lung carcinoma.