Of the 72 distinct EGFR variants, 15 of them (single or complex EGFR mutations) were newly identified in NSCLC (11 of them detected by NGS, 2 of them detected by Sanger sequencing and 3 detected by real-time PCR, with 1 detected by both Sanger sequencing and NGS). The gene discussed is EGFR; the disease is non-small cell lung carcinoma.