EGFR and non-small cell lung carcinoma: Of the 21,324 NSCLC samples successfully tested for EGFR, 9,621 of them carried somatic EGFR mutations, representing an average positive EGFR detection rate of 45.1% including 40.3% (2,111/5,244) tested by Sanger sequencing, 46.5% (6,202/13,329) tested by real-time PCR, and 47.5% (1,308/2,751) tested by NGS respectively (Figure 1A).