The median EGFR VAFs were 0.074 (range: 0.01–0.675), 0.097 (range: 0.011–0.97), 0.161 (range: 0.01–0.902), 0.254 (range: 0.014–0.811), and 0.261 (range: 0.01–0.957) for NSCLC samples with 1, 5, 10, 15, and ≥20% TCC respectively. Here, EGFR is linked to non-small cell lung carcinoma.