To further explore the hub genes protein expression in HCC, we analyzed immunohistochemistry staining images about CDK1 (five samples), CCNB1(seven samples), AURKA (seven samples), CCNA2(six samples), KIF11(6 samples), BUB1B(none), TOP2A (7 samples), TPX2 (6 samples), HMMR (3 samples) and CDC45 (8 samples) in HCC tissues and normal tissues from the HPA(Figure 8). This evidence concerns the gene BUB1B and hepatocellular carcinoma.