In addition, the inhibition of LRIG1 expression recapitulated the trastuzumab resistance phenotype, decreased cell apoptosis rate and activated downstream AKT and ERK signal after treatment with trastuzumab in ER stressed GC cells with stably silenced miR-301a-3p (Fig. 4f–h, Supplementary Fig. S4e). Here, AKT1 is linked to gastric cancer.