RUNX1 and Hyperglycemia: Last, to further explore a possible causal role for RUNX1 in hyperglycemia-induced trained immunity responses, BMDMs that had previously been differentiated in physiological or high glucose were restored to 5 mmol/L glucose and then stimulated in the absence or presence of the pharmacological RUNX1-specific inhibitor Ro5-333551 (Figure 8D).