Whether the differential frequencies of above clonal neoantigenic mutations were associated with prognosis of Pt204, the mean frequencies of 4 neoantigenic mutations (KRAS p.G12V, TFR2 p.R771H, TNR p.V577I, ZBTB5 p.V41M) from the trunk in primary lesion and 6 metastatic tumors were calculated as neoantigenic clonal fraction (NCF) for Pt204’s each sample. This evidence concerns the gene ZBTB5 and metastatic neoplasm.