As shown in Figure S2 and Fig. 3f, there were obvious higher level of CD3, CD8 or CD4 positive tumor infiltrating lymphocytes in stroma region of primary lesion in Pt295 and Pt540 rather than those in Pt204, while the CD68+ macrophages and CD68+CD163−macrophages were enriched in stroma region of Pt204 and almost not present in those of Pt295 and Pt540. This evidence concerns the gene CD8A and neoplasm.