Furthermore, from D1 vs D2 comparisons (Supplementary Fig. 7, Supplementary Table. 2, 9, 10), we also found Shisa6 in the D1 Sus DEG list, which is associated with highly relevant GO terms such as MDD, bipolar disorder, and anxiety in a GWAS database (Supplementary Fig. 7d). The gene discussed is SHISA6; the disease is major depressive disorder.