Because we observed, several of the genes that were downregulated after EPZ-5676 treatment in ovarian cancer cells are associated with cellular biosynthesis pathways such as macromolecule biosynthesis, RNA biosynthesis, organ development, skeletal system morphogenesis, and stem cell division we performed a large-scale unbiased metabolomic analysis with the goal of understanding the specific mechanism of DOT1L action. This evidence concerns the gene DOT1L and ovarian carcinoma.