HCT116 ATG16L1 T300 colon cancer cells and GBM6 cells, a PDX glioblastoma isolate expressing truncated EGFR vIII, were transfected with a plasmid to express LC3-GFP-RFP and with siRNA molecules to knock down expression of AMPKα1 or eIF2α. Here, ATG16L1 is linked to malignant colon neoplasm.