SPHK1 and neoplasm: The expression of 213 ARGs was analyzed in 79 EAC and 9 non-tumor tissues using ‘limma’ package in R, and 21 differentially expressed ARGs were eventually screened with the criteria of |log2FC| > 1.5 and FDR < 0.05, including 18 upregulated genes (BIRC5, CDKN2A, IL24, SERPINA1, CASP1, BAX, BID, IFNG, IKBKE, TP73, ATG9B, TNFSF10, APOL1, SPHK1, ATIC, RGS19, ITGB4, ITGA3) and 3 downregulated genes (MAP1LC3C, NKX2-3, PARK2) (Figure 1a-b).