MDA‐MB‐231 cells were selected as a model of breast cancer cells known for producing a robust invadopodial response at matrix contact sites.[20] When plated on fluorescently labeled gelatin for 60 min in complete medium (CM), several gelatin degradation spots were visible underneath the cells, which coincided with punctate invadopodia positive for TKS5 (endogenous or overexpressed GFP‐tagged protein) located underneath or in the vicinity of the nucleus (Figure 1a and Figure S1a, Supporting Information). Here, SH3PXD2A is linked to breast cancer.