Leptin was increased in patients with severe liver steatosis (stage 0 vs. 3, p < 0.05) (Figure 1D) and tended to increase with lobular inflammation and hepatic ballooning, while no significant differences were found with other co-morbidities, fibrosis stage, or NAFLD-associated polymorphisms (Supplementary Figure 1). Here, LEP is linked to fatty liver disease.