Next, we investigated the effect of hAM homogenate on the expression of cyclin D1, which is essential for G1/S phase transition and its overexpression had been recorded in a large proportion of human cancers, including bladder cancer (Tut et al., 2001; Musgrove et al., 2011; Choi et al., 2012; Kopparapu et al., 2013). Here, CCND1 is linked to urinary bladder cancer.