Significant differences between genetic expression of immune mediators were detected at 72h but not at 24h post infection with L. interrogans, except for an increase of IP-10 between L. interrogans and control (Table 1) at 24h, and a decrease in IL-1β between L. biflexa and L. interrogans infected mice (Figure 4E) at 24h. This evidence concerns the gene CXCL10 and infection.