Moreover, cell cycle arrest serves as a primary mechanism for inducing apoptosis and flow cytometry analysis showed that NRP1 knockdown caused a significant decrease in the percentage of cells in the G0/G1 peak, and an increase in the percentage of cells in the G2/M peak, however statistically significant changes were not observed in the S peak (Figure 3B), indicating that NRP1 may promote proliferation in BC cells by reducing apoptosis through mediating the G0/G1 and G2/M phase transitions. This evidence concerns the gene NRP1 and breast cancer.