Previously knockout of such types of retrogenes, including Cetn1, Cstf2t, Pgk2 and Rpl10l, always resulted in spermatogenic abnormalities and male infertility (Dass et al., 2007; Danshina et al., 2010; Tardif et al., 2010; Avasthi et al., 2013; Jiang et al., 2017). This evidence concerns the gene PGK2 and male infertility.