These CAR-T cells include 1) CAR-T cells that target several cancer antigens simultaneously, such as CD19 and CD22, which prevents antigen loss (255), 2) CAR-T cells that directly suppress immune checkpoints (254), 3) CAR-T cells that use gene editing to insert the CAR gene in a position that enhances activation and reduces T cell exhaustion (256), 4) CAR-T-cells secreting cytokines such as IL-12 which may impair the suppressive function of the tumor microenvironment (257, 258). The gene discussed is CD19; the disease is neoplasm.