Our in vitro experiments that mixed mesenteric and draining lymph node cells demonstrated that the production of IFN-γ and IL-10 is dependent on TLR2, LAP and IL-10, since the monoclonal antibodies neutralizing these molecules induced increased IFN-γ and decreased IL-10 production, confirming previous data found in models of colitis and arthritis (17, 66). This evidence concerns the gene IFNG and arthritic joint disease.