•In ALS's spinal cord TDP-43 (N-terminal and C-terminal) labeled in NCIs but in FTLD-TDP's hippocampus only C-terminal of TDP-43 were labeled.•Double-labeling of PABP-1 and pTDP-43 of the same cases showed positive inclusions in spinal cord but not in cortical inclusions. The gene discussed is TARDBP; the disease is amyotrophic lateral sclerosis.