The differential proteins between EBV-positive and negative GC were found to be significantly expressed in numerous cell lines and tissue such as H293 cell (P = 1.23E-14), CaOV3 cell (P = 9.47E-14), CD8 cell (P = 8.19E-13), ascites cancer cell (P = 1.98E-12) and colorectal cancer (CRC) tissue (P = 6.02E-12). This evidence concerns the gene CD8A and colorectal cancer.