Transwell assays displayed that the number of migrated cells obviously was reduced after the transfection of sh/NCK1-AS1#1/2 into melanoma cells, while the transfection of pcDNA3.1/NCK1-AS1 led to an increase on the number of migrated cells (Fig. 1F, Additional file 1: Figure S1E). Here, NCK1 is linked to melanoma.