To investigate the loss-of-function or gain-of-function effects of NCK1-AS1 in melanoma, we transfected plasmids of sh/NCK1-AS1#1/2/3 or pcDNA3.1/NCK1-AS1 into melanoma cells to knock down or overexpress NCK1-AS1 (Fig. 1B; Additional file 1: Figure S1A). The gene discussed is NCK1; the disease is melanoma.