Meanwhile, the apoptosis of neuroblastoma cells was analyzed using AnnexinV-FITC/PI staining and flow cytometry as depicted in Fig. 5G. The percentages of early apoptotic cells were decreased from 5.18 to 3.18%, and late apoptotic cells were dropped from 1.02 to 0.58% compared with the MPP+-treated SH-SY5Y cells which was transfected with pcDNA3.1. Here, ANXA5 is linked to neuroblastoma.