In this study, we aim to determine the expression levels of ACE, ELMO1, and WT1 mRNAs in the blood extracellular vesicles (EVs) of DN patients and diabetic patients without nephropathy (DM patients) compared to healthy controls and investigate whether or not the quantifications of these mRNAs are associated with the degree of albuminuria and the severity of glomerular injuries. Here, ACE is linked to Nephropathy.