We showed that purified scEDIII from recombinant yeast induced a balanced immune response in mice and that when it was conjugated with gut mucosal layer binding ligands such as Co1 or LTB, it had immense potential as a recombinant tetravalent Dengue oral vaccine (Nguyen et al. 2013; Bal et al. 2018a, b). Here, LTB is linked to dengue disease.