sgRNAs specifically targeting BIRC3 (exon 2) were then introduced in HG3-del(11q) cells in order to induce BIRC3 truncating mutations (BIRC3MUT) in the remaining WT allele, generating HG3-del(11q) BIRC3MUT isogenic cell lines (Fig. 1A), mimicking the BIRC3 biallelic loss through del(11q) and mutation observed in high-risk CLL patients. The gene discussed is BIRC3; the disease is B-cell chronic lymphocytic leukemia.