Additionally, double immunohistochemical evaluation using antibodies against p62 and TDP-43 on five different sections at the fourth level of the lumbar cord to determine the cytopathological coexistence of motoneuronal p62-positive intranuclear inclusions (NIID pathology) and pTDP-43-positive cytoplasmic inclusions with native TDP-43 loss from the nucleus (ALS pathology) did not show any motor neuron overlap between NIID and ALS pathology (Fig. 6). This evidence concerns the gene SQSTM1 and amyotrophic lateral sclerosis.