In total, TP53, MED16, DRC7, CEND1, ATAD5, SETD8, and PIK3CA were identified as significantly mutated genes (SMGs, Supplementary Table 7) in the 13 MIBC samples, and 13 key genes associated with the tumorigenesis of BC were illustrated in a heat map (Figure 1B). The gene discussed is PIK3CA; the disease is breast cancer.