The analysis of the gene expression levels of SASP components Tgfβ1, Il6, Ctgf/Ccn2 and Serpine1 (which encodes PAI-1) and the IL-6 protein levels assayed by ELISA, in the early phase of AKI, showed that all of the evaluated SASP components were higher in old AKI mice compared to the young ones (Figures 8A–E). This evidence concerns the gene CCN2 and acute kidney injury.