To determine the role of Syncytin-1 on doxorubicin-mediated HCC cell apoptosis, Huh7 cells, which with the lower expression level of Syncytin-1, were transfected with pCMV-Syncytin-1 plasmids followed by treatment with doxorubicin. This evidence concerns the gene ERVW-1 and hepatocellular carcinoma.