To investigate whether GL25E could inhibit the infection of SARS-CoV-2 mutants, we generated 5 SARS-CoV-2 PsVs with single amino acid mutation in S protein, including V341I, R408I, G476S, V483A and N501Y, and used them to test the effect of these mutations on the inhibitory activity of GL25E on SARS-CoV-2 PsV infection. The gene discussed is PROS1; the disease is infection.