Our results suggest that eRNA TBX5-AS1 might exert its antitumour effects through the following way: TBX5-AS1 may regulate chromatin loop stability by binding to specific protein factors, which in turn promotes the expression of its target gene TBX5. And then, TBX5 suppress the invasion lung adenocarcinoma by affecting PI3K/AKT pathway, Ras signaling pathway, cGMP−PKG signaling pathway, MAPK signaling pathway, etc. In summary, the present findings suggested that TBX5-AS1 has potential as a diagnostic biomarker and therapeutic target for LUAD. Here, TBX5 is linked to lung adenocarcinoma.