Use of two independent shRNAs (-sh1,-sh2) to deplete MSI2, versus empty vector, in two human CRC cell lines (RKO and HCT116), significantly decreased TGFβ1, CDH1, and ZO-1 protein expression in both cell lines, while not affecting mRNA levels for these proteins (Fig 4A–4D). This evidence concerns the gene TJP1 and colorectal carcinoma.